13 Jul 2023
 | 13 Jul 2023
Status: this preprint is currently under review for the journal SP.

Laboratory experiments in ocean alkalinity enhancement research

Maria Debora Iglesias-Rodríguez, Rosalind E. M. Rickaby, Arvind Singh, and James A. Gately

Abstract. Recent concern about the consequences of continuing increases in atmospheric CO2 as a key heat-trapping agent (USGCRP, 2017; IPCC, 2021) have prompted ocean experts to come together to discuss how to provide science-based solutions. Ocean alkalinity enhancement (OAE) is being considered not only as a ocean carbon dioxide removal (CDR) approach, but also as a potential way to mitigate ocean acidification. Over the last two decades, inter-laboratory comparisons have proven valuable in evaluating the reliability of methodologies associated with sampling and analysis of carbonate chemistry parameters, which have been routinely used in ocean acidification research (Bockmon and Dickson, 2015). Given the complexity of processes and mechanisms related to ecosystem responses to OAE, consolidating protocols to ensure compatibility across studies is fundamental for synthesis and upscaling analysis. This chapter provides an overview of best practice in OAE laboratory experimentation and facilitates awareness of the importance of applying standardized methods to promote data re- use, inter-lab comparisons, and transparency. This chapter provides the reader with the tools to (1) identify the criteria to achieve the best laboratory practice and experimental design; (2) provide guidance on the selection of response variables for various purposes (physiological, biogeochemical, ecological, evolutionary) for inter-lab comparisons; (3) offer recommendation for a minimum set of variables that should be sampled and propose additional variables critical for different types of synthesis and upscaling; and (4) identify protocols for standardized measurements of response variables.

Maria Debora Iglesias-Rodríguez et al.

Status: final response (author comments only)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on sp-2023-7', Anonymous Referee #1, 07 Aug 2023
  • RC2: 'Comment on sp-2023-7', Alex Poulton, 24 Aug 2023
  • RC3: 'Comment on sp-2023-7', Justin Ries, 26 Aug 2023

Maria Debora Iglesias-Rodríguez et al.

Maria Debora Iglesias-Rodríguez et al.


Total article views: 405 (including HTML, PDF, and XML)
HTML PDF XML Total BibTeX EndNote
253 139 13 405 5 13
  • HTML: 253
  • PDF: 139
  • XML: 13
  • Total: 405
  • BibTeX: 5
  • EndNote: 13
Views and downloads (calculated since 13 Jul 2023)
Cumulative views and downloads (calculated since 13 Jul 2023)

Viewed (geographical distribution)

Total article views: 399 (including HTML, PDF, and XML) Thereof 399 with geography defined and 0 with unknown origin.
Country # Views %
  • 1
Latest update: 29 Sep 2023
Short summary
This chapter provides an overview of best practice in ocean alkalinity enhancement (OAE) laboratory experimentation by identifying the criteria to achieve the best laboratory practice, providing guidance on which parameters to measure to capture important chemical and biological responses and identifying additional parameters to conduct inter-lab comparisons. The guidance provided in this chapter is critical to in order to gain critical information to move OAE research to the field.